Coding

Part:BBa_K4960039:Design

Designed by: Ziying Wang   Group: iGEM23_NUDT-CHINA   (2023-10-11)


pPVC promoter->pvc1-pvc12->pvc13_NTD-3*EAAAK-CKGGRAKDC-3*EAAAK-pVc13_CT->pvc14-pvc16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2407
    Illegal BglII site found at 11264
    Illegal BglII site found at 14099
    Illegal BglII site found at 17020
    Illegal XhoI site found at 2691
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 11792
    Illegal NgoMIV site found at 17551
    Illegal NgoMIV site found at 17690
    Illegal AgeI site found at 6704
    Illegal AgeI site found at 9641
    Illegal AgeI site found at 10327
    Illegal AgeI site found at 10468
    Illegal AgeI site found at 11576
    Illegal AgeI site found at 17989
    Illegal AgeI site found at 19677
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A


Source

A

References